Ultrastructure of Single Cells, Callus-like and Monosore-like Cells in Porphyra yezoensis Ueda on Semi solid Culture Med

It had been demonstrated that individual cells or protoplasts isolated from Porphyra thallus by enzyme could develop into normal leafy thalli in the same way as monospores, and that isolated cells develop in different way in liquid and on semi solid media. The authors observed the ultrastructure of isolated vegetative cells cultured on semi solid media and compared them with those of monospores and isolated cells cultured in liquid media. The results showed that subcellular structures were quite different among cells in different conditions. In their development, isolated cells on semi solid media did not show the characteristic subcellular feature of monospore formation, such as production of fibrous vesicles. Callus like cells formed on semi solid media underwent a distinctive modification in cellular organization. They developed characteristic cell inclusions and a special 2 layer cell covering. Golgi bodies, ER, starch grains, mitochondria. Vacuoles were not commonly found in them.

作 者： 梅俊學 沈頌東 姜明 費修綆   作者單位： 梅俊學(Marine Biotechnology Department, Shandong University Weihai Branch, Weihai 264209, China)

沈頌東(Biology Department, Suzhou University West Campus, Suzhou 215151, China)

姜明(Qingdao Ocean University, Qingdao 266003, China)

費修綆(Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071,China) 

刊 名： 中國海洋湖沼學報(英文版)  ISTIC SCI 英文刊名： CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY  年，卷(期)： 2003 21(2)  分類號： Q17  關(guān)鍵詞： cell   monospore   nori   Porphyra yezoensis   protoplast   ultrastructure  

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