Isolation and characterization of rice lesion mimic mutants from a T-DNA tagged population

A rice ( Oryza sativa L. ssp. japonica cv. Nipponbare) T-DNA tagged population consisting of about 7000 individual lines was generated and screened for rice lesion mimic mutants in the T1 generation. Ten lines were found to develop spontaneous lesions in the absence of pathogen infection and displayed distinct lesion phenotypes. These mutants were tentatively designated as lm1 -lm10 (for lesion mimic), respectively. Lesion formation of lm mutants was developmentally regulated, and all the mutants showed stunted growth and reduced fertility. Genetic analysis demonstrated that all the mutations were recessive, and five partially fertile mutants (lm4-lm8) were derived from different loci. Mimic lesions occurring on the leaves of lm mutants resulted from cell death as revealed by trypan blue staining. Six of them ( lm3 -lm8 ) exhibited enhanced resistance to five bacterial blight isolates, indicating their wide-spectrum resistance to this pathogen. These results imply that some lesion mimic mutations of rice might be involved in disease resistance signaling pathways,and that isolation of these mutated genes may be useful for elucidating molecular mechanisms of plant disease resistance. Among the mutants, only one mutant, lm6, was preliminarily shown to cosegregate with the inserted T-DNA in its T1 generation, making it feasible to isolate the gene responsible for the phenotype of this mutant.

作 者： Li Shutian PEI Zhongyou Luo Lijuan TIAN Yingchuan HE Chaozu   作者單位： Li Shutian(National Key Laboratory of Plant Genomics, Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China;Graduate School of the Chinese Academy of Sciences, Beijing 100039, China)

PEI Zhongyou,TIAN Yingchuan,HE Chaozu(National Key Laboratory of Plant Genomics, Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China)

Luo Lijuan(College of Agronomy, South China University ofTropical Agriculture, Danzhou 571737, China) 

刊 名： 自然科學(xué)進展（英文版）  SCI 英文刊名： PROGRESS IN NATURAL SCIENCE  年，卷(期)： 2005 15(1)  分類號： Q3  關(guān)鍵詞： T-DNA tagging   lesion mimic   hypersensitive response   Xanthomonas oryzae pv. Oryzae   Oryza sativa  

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