久久99热66热这里只有精品,特黄特色的大片在线观看,亚洲日本三级在线观看,国产三级农村妇女在线,亚洲av毛片免费在线观看,哺乳叫自慰在线看,天天干美女av网

厭氧發(fā)酵過(guò)程pH對(duì)微生物多樣性和產(chǎn)物分布的影響

時(shí)間:2023-04-25 20:15:49 環(huán)境保護(hù)論文 我要投稿
  • 相關(guān)推薦

厭氧發(fā)酵過(guò)程pH對(duì)微生物多樣性和產(chǎn)物分布的影響

摘要: 采用PCR-DGGE技術(shù),研究了不同pH條件下蔬菜類(lèi)有機(jī)垃圾厭氧發(fā)酵過(guò)程中的微生物多樣性,探討了微生物群落結(jié)構(gòu)與發(fā)酵產(chǎn)物分布的關(guān)系.Shannon指數(shù)分析表明,pH=7和pH=8時(shí)的微生物多樣性較高,隨時(shí)間變化規(guī)律相似,而pH=5時(shí)的微生物多樣性較低.UPGMA聚類(lèi)分析和PCA分析結(jié)果也表明,pH=7和pH=8時(shí)的微生物群落結(jié)構(gòu)相似,pH=5與之顯著不同.在不同pH條件下的優(yōu)勢(shì)菌屬都是乳酸細(xì)菌和梭菌.微生物多樣性的變化與發(fā)酵產(chǎn)物分布具有一定相關(guān)性,pH通過(guò)影響微生物群落結(jié)構(gòu)的變化最終影響發(fā)酵產(chǎn)物的分布.圖4表2參27 Abstract: pH is the prominent parameter influencing the product distribution of anaerobic fermentation where bacteria play a key role. To estimate the effect of pH (5, 7 and 8) on bacterial diversity and correlation between product distribution and bacterial community structure during the fermentation of vegetable waste, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR -DGGE) was applied. Three statistical methods were used to analyze the DGGE profiles. Shannon diversity index revealed that the bacterial diversity at pH = 5 was a bit lower than that at pH = 7 or pH = 8. Dendrograms calculated with unweighted pair group method with arithmetic mean (UPGMA) indicated a close similarity of bacterial communities between pH = 7 and pH = 8, while the bacterial community at pH = 5 differed. Analysis based on primary component analysis (PCA)presented a consistent conclusion but more detailed with information about bacterial evolution. According to the results of sequencing, lactic acid bacteria and Clostridium sp. were predominant at all pH values. Bacterial community during fermentation was materially influenced by pH which finally caused the diverse product distributions of fermentation. Fig 4, Tab 2, Ref 27 作 者: 葉凝芳    何品晶    呂凡    邵立明    YE Ningfang    HE Pinjing    L(U) Fan    SHAO Liming   作者單位: 同濟(jì)大學(xué)污染控制與資源化研究國(guó)家重點(diǎn)實(shí)驗(yàn)室,上海,200092  期 刊: 應(yīng)用與環(huán)境生物學(xué)報(bào)  ISTICPKU   Journal: CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY  年,卷(期): 2007, 13(2)  分類(lèi)號(hào): X172 X705  關(guān)鍵詞: 微生物多樣性    pH    厭氧發(fā)酵    發(fā)酵產(chǎn)物分布    變性梯度凝膠電泳    Keywords: microbial diversity    pH    anaerobic fermentation    product distribution    denaturing gradient gel electrophoresis   

【厭氧發(fā)酵過(guò)程pH對(duì)微生物多樣性和產(chǎn)物分布的影響】相關(guān)文章:

《降水和降水的分布》教案02-25

《細(xì)菌和真菌的分布》教案03-09

《細(xì)菌和真菌的分布》教學(xué)反思(通用6篇)09-28

潮汐和海風(fēng)對(duì)海釣的影響09-16

細(xì)胞的多樣性和統(tǒng)一性的教案04-25

高中化學(xué)-高二水的電離和溶液的pH值教案12-30

果酸水楊酸與pH值12-20

血液的組成成分和產(chǎn)生過(guò)程05-17

網(wǎng)球的適宜人群和影響10-18

復(fù)工復(fù)產(chǎn)物資設(shè)備總結(jié)04-07